Studies on the purification of DNA polymerase α and the control mechanism of enzyme activity in NIH3T3 cells = NIH3T3 세포내 DNA 중합효소의 순수분리 및 효소 활성도의 조절 기작에 관한 연구

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It has been known that DNA polymerase a plays $\alpha$ major role in DNA replication of eukaryotes, moreover has function of repairing damaged DNA. To purify DNA polymerase $\alpha$, two different protocols were used;First was conventional chromatographic method that has been generally used for protein purification, Second was immunoaffinity chromatography using monoclonal antibody which specifically binds to human DNA polymerase $\alpha$. Of purified DNA polymerase $\alpha$ subunits, $>$190kDa protein was degraded by protease. Its activity was reduced by aphidicolin, specific DNA polymerase $\alpha$ inhibitor, but not by dideoxythymidine triphosphate which is an inhibitor of other DNA polymerases. DNA polymerase a activity in cell was changed during the progression of cell cycle. Enzyme activity was increased slightly before DNA replication(S phase). When NIH3T3 cells were irradiated by UV light to damage chromosomal DNA, DNA polymerase $\alpha$ activity was increased in nuclear fraction. When cell were treated with PMA(Phorbol myristate acetate), protein kinase C activator and modulator of cell division and differentiation, DNA polymerase $\alpha$ activity increased in cytoplasmic and nuclear fractions. DNA polymerase $\alpha$ is involved in DNA repair and DNA replicaiton, and the increment of enzyme activity is a result of posttrannnslational modification. This implicates that DNA polymerase $\alpha$ activity might be controlled by protein kinase C in vivo.
Advisors
Kang, Ke-WonJoe, Cheol-O강계원조철오
Description
한국과학기술원 : 생물공학과,
Publisher
한국과학기술원
Issue Date
1993
Identifier
68327/325007 / 000911640
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물공학과, 1993, [ iv, 48 p. ]

Keywords

단백질 분리.

URI
http://hdl.handle.net/10203/28453
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=68327&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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