Transport of inositol 1,4,5-triphosphate through gap junction channels : a possible mode of intercellular communication of second messengers = 간극 연접을 통한 이노시톨 1,4,5-트리스인산의 이동 : 세포간 이차 신호물질의 전달방법에 대한 가설a possible mode of intercellular communication of second messengers

Cited 0 time in webofscience Cited 0 time in scopus
  • Hit : 347
  • Download : 0
This study was conducted to assess the effects of chemical carcinogens on the gap junctional intercellular communication in cultured mammalian cells and to prove that gap junction channel provide a pathway for the calcium-mobilizing second messenger, inositol 1,4,5-trisphosphate ($IP_3$). Clone 9 cells derived from rat liver were treated with various carcinogens and anticancer drugs. Gap junctional communication was assessed by measuring the transfer of scrape-loaded lucifer yellow dye. Direct carcinogens such as N-methyl-N``-nitro-N-nitrosoguanidine, hydroxylamine and ethidium bromide exhibited strong inhibition of intercellular communication, while indirect carcinogens such as aflatoxin $B_1$ and ethionine exerted minor effects. The passage of second messenger molecules through gap junction channels was proved by the experiment in which scrape-loaded [$^3H$]-$IP_3$ was transferred into cells along the scraped line. This spread of labled second messenger into neighboring cells was visualized by microautoradiography. To show the direct involvement of gap junction channel in the transport of second messenger, channels were formed in artificial unilamellar liposomes using connexin 32 gap junction protein immunoaffinity purified from rat liver. $[^3H]-IP_3$ was incorporated into vesicles containing open gap junction protein but the vesicles with closed channels failed to incorporate $[^3H]-IP_3$.
Advisors
Joe, Cheol-Oresearcher조철오researcher
Description
한국과학기술원 : 생명과학과,
Publisher
한국과학기술원
Issue Date
1995
Identifier
98687/325007 / 000933157
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생명과학과, 1995.2, [ vii, 65 p. ]

Keywords

세포 신호 전달

URI
http://hdl.handle.net/10203/28437
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=98687&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
Files in This Item
There are no files associated with this item.

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0