NTPase activity of hevatitis C virus NS3 protein expressed in insect cells = 곤충 세포에서 발현된 C형 간염 바이러스 NS3단백질의 NTPase 활성에 관한 연구

Cited 0 time in webofscience Cited 0 time in scopus
  • Hit : 282
  • Download : 0
The genomic RNA of Hepatitis C virus (HCV) contains a single large open reading frame for virion structural proteins and viral nonstructural polypeptides. HCV NS3 protein (p70) was predicted to be a serine-type proteinase, a nucleotide triphosphatase (NTPase), and an RNA helicase based on the presence of sequence motifs. We demonstrated that the NS3 protein expressed in insect cells possesses an NTPase activity. Employing the baculovirus system, we partially purified the NS3 protein fused to 6-histidine residues using metal-binding chromatography. It is revealed that an analog of p70, p47 was also expressed. The p47 protein did not seem to contain an N-terminal serine protease domain of the p70 protein, because p47 did not bind to a metal-binding column. The elutes containing p70 showed a specific NTPase activity. This activity was not found in the other elute fractions. NTPase activity was completely inhibited by the murine NS3 specific antibodies. Above results strongly suggest that NS3 protein of HCV contains NTPase activity.
Advisors
Choe, Joon-Horesearcher최준호researcher
Description
한국과학기술원 : 생명과학과,
Publisher
한국과학기술원
Issue Date
1995
Identifier
98670/325007 / 000933021
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생명과학과, 1995.2, [ iv, 40 p. ]

Keywords

C형 간염 바이러스

URI
http://hdl.handle.net/10203/28431
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=98670&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
Files in This Item
There are no files associated with this item.

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0