Molecular cloning of ras homologous regions from tobacco(Nicotiana tabacum) genomic DNA library = 담배(Nicotiana tabacum)의 genomic DNA library로 부터 ras 상동부위의 molecular cloning

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The ras gene plays an important role in signal transduction in eukaryotes. Isolation of ras or ras-related gene from Nicotiana tabacum was attempted. Genomic DNA was screened with human c-Ha-ras as a probe and 15 positive clones were obtained. Each clone was classified into three subgroups according to its signal intensity and three clones were selected from each group. DNA was purified from those clones and digested with many types of restriction endonucleases. Southern analysis was carried out and three DNA fragments of group I and II which showed positive signal was subcloned into cloning-sequencing vector, pBluscriptIIKS+. Cloned DNA fragments were restricton endonuclease mapped.
Advisors
Chung, Won-Ilresearcher정원일researcher
Description
한국과학기술원 : 생명과학과,
Publisher
한국과학기술원
Issue Date
1994
Identifier
69130/325007 / 000923345
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생명과학과, 1994.2, [ iv, 36 p. ]

URI
http://hdl.handle.net/10203/28423
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=69130&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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