DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Byun, Si-Myung | - |
dc.contributor.advisor | 변시명 | - |
dc.contributor.author | Bae, Kwang-Hee | - |
dc.contributor.author | 배광희 | - |
dc.date.accessioned | 2011-12-12T08:59:43Z | - |
dc.date.available | 2011-12-12T08:59:43Z | - |
dc.date.issued | 1994 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=69126&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/28419 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 생명과학과, 1994.2, [ vi, 49 p. ] | - |
dc.description.abstract | Ser-49 in subtilisin J, an extracellular serine protease from B. stearothermophilus, is highly conserved residue among subtilisins from Bacillus sp. Moreover, Ala-48 - Ser-49 peptide bond was known as a primary autolysis site in subtilisin BPN`` recently. Previously constructed Asp-49 mutant protein, corresponding to that a more thermostable subtilisin variants, shows proteolytic activity, but so unstable that it was degraded during cultivation in Tyr-58 - Gln-59 peptide bond. The enzymatic activity of the Asp-49 mutant protein is higher than that of wild-type in early growth phase. To elucidate the role of Ser-49 and increase the heat-stability, first, the Ser-49 was replaced with Thr,Asn and Glu. From results of substitution of Ser-49, we concluded that hydroxyl of Ser-49, as H-bond donor and acceptor, is important in prevention of autolysis. Second, in consideration of substrate specificity of subtilisin, The Tyr-58 or Gln-59 weae replaced with various amino acid using Asp-49 mutant as a template. Among the mutants, Asp-49 -$\Delta$Tyr-58 mutant showed a considerably improved resistance to heat. These results demonstrate the powerful probability of protein-engineering in enhancement of heat stability of various enzymes. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | 부위 특이 돌연변이 유발. | - |
dc.title | Improvement of heat-stability of subtilisin J by protein-engineering | - |
dc.title.alternative | 단백질 공학을 이용한 서브틸리신 J의 열안정성 증가 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 69126/325007 | - |
dc.description.department | 한국과학기술원 : 생명과학과, | - |
dc.identifier.uid | 000923217 | - |
dc.contributor.localauthor | Byun, Si-Myung | - |
dc.contributor.localauthor | 변시명 | - |
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