DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Yoo, Ook-Joon | - |
dc.contributor.advisor | 유욱준 | - |
dc.contributor.author | Kang, Jong-Seok | - |
dc.contributor.author | 강종석 | - |
dc.date.accessioned | 2011-12-12T08:59:36Z | - |
dc.date.available | 2011-12-12T08:59:36Z | - |
dc.date.issued | 1994 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=69118&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/28411 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 생명과학과, 1994.2, [ v, 57 p. ] | - |
dc.description.abstract | As a first step toward understanding regulation of the phosphatidylinositol - specific phospholipase C(PLC) isoform genes, the 5`` flanking region of the Rat PLC-$\beta$3 gene was sequenced and characterized. Sequence analysis of the 5`` flanking region revealed that it contains a CCAAT sequence at position -289 and a extremely high GC- rich region(-305 to -16bp: 78\%). But, TATA box was not found in this region. This 5`` region also contained binding sites for the transcription factors Sp1, AP-1, AP-2, and PEA3. When the putative promoter was ligated into a promoterless CAT vector and transfected into C$_6$Bu1 cells, CAT activity was expressed, indicating the promoter region was located in this fragment. To define sequences that influence transcription of the PLC-$\beta$3 gene, transcription activities of various 5`` deletion mutants of its upstream region, fused to the CAT gene, were examined in terms of CAT expression after transfection into C$_6$Bu1 cells. Deletion mutational analysis revealed that the PLC-$\beta$3 promoter contains at least three positive elements; -1827 to -1543 bp, -1232 to -1072 bp, and -587 to -489 bp. In addition to these positive elements, there are two negative elements; -1543 to -1393 bp and -643 to -587 bp. Gel mobility shift assays revealed that these elements form multiple protein- DNA complexes with nuclear proteins in C$_6$Bu1 cells. These results suggest the presence of multiple trans - acting factors that act on these positive and negative cis - acting elements and regulate the transcription of the PLC-$\beta$3 gene. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.title | Characterization of the 5'-flanking region of the rat phospholipase C β3 gene | - |
dc.title.alternative | 쥐 포스포라이페이즈 C 베타3 유전자의 5'말단 부위에 대한 분석 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 69118/325007 | - |
dc.description.department | 한국과학기술원 : 생명과학과, | - |
dc.identifier.uid | 000923009 | - |
dc.contributor.localauthor | Yoo, Ook-Joon | - |
dc.contributor.localauthor | 유욱준 | - |
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