DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Jung, Ki Jun | - |
dc.contributor.advisor | 정기준 | - |
dc.contributor.author | Sohn, Min Gi | - |
dc.date.accessioned | 2021-05-12T19:37:32Z | - |
dc.date.available | 2021-05-12T19:37:32Z | - |
dc.date.issued | 2020 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=910856&flag=dissertation | en_US |
dc.identifier.uri | http://hdl.handle.net/10203/284052 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 생명화학공학과, 2020.2,[iv, 54 p. :] | - |
dc.description.abstract | In the production of amino acids, biofuels, and pharmaceuticals using microbial fermentation, maintaining the productivity of cells even during industrial scale of long-term fermentation is an important subject that is continuously studied. Methionine is essential for life for the synthesis of protein. Since chemical synthesis requires hazardous chemicals and is obtained as racemic compound (DL-methionine), research on the production of L-methionine using microbial fermentation is becoming attractive. In this study, growth-based methionine biosensor was developed to maintain the productivity of L-methionine by selection of low-producers, when Corynebacterium glutamicum produced L-methionine. Essential genes for cell growth were combined with transcription factors and promoters that recognize and operate by methionine, and it allows genes to be expressed so that only strains that produce methionine can be selectively grown. | - |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | industrial sacle▼along-term fermentation▼aproductivity▼amethionine▼aCorynbebacterium glutamicum▼agrowth-based biosensor | - |
dc.subject | 산업 스케일▼a생산성 유지▼a메티오닌▼a코리네박테리움 글루타미쿰▼a세포 성장 기반 바이오 센서 | - |
dc.title | Development of genetic circuits for enhanced production of methionine in corynebacterium glutamicum | - |
dc.title.alternative | 코리네박테리움 글루타미쿰에서 메티오닌 생산증대를 위한 유전자 회로 제작 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 325007 | - |
dc.description.department | 한국과학기술원 :생명화학공학과, | - |
dc.contributor.alternativeauthor | 손민지 | - |
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