Synthetic ribozyme suppressing HBV X gene expression = 합성된 리보핵산효소에 의한 HBV X 유전자 발현 억제

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dc.contributor.advisorKang, Chang-Won-
dc.contributor.advisor강창원-
dc.contributor.authorLee, Bum-Yong-
dc.contributor.author이범용-
dc.date.accessioned2011-12-12T08:59:27Z-
dc.date.available2011-12-12T08:59:27Z-
dc.date.issued1993-
dc.identifier.urihttp://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=68313&flag=dissertation-
dc.identifier.urihttp://hdl.handle.net/10203/28401-
dc.description학위논문(석사) - 한국과학기술원 : 생명과학과, 1993.2, [ vi, 47 p. ]-
dc.description.abstractRibozymes are potentially very powerful agents for perturbing intracellular gene expression. Hammerhead structure ribozymes were designed to cleave the mRNA of hepatitis B virus X gene. Its cleavage was designed to result in the suppressed expression of lacZ fused to a 5$^\prime$462-bp fragment of X gene. The hammerhead ribozymes consist of a core region for cleavage activity and two variable regions for binding to a target RNA sequenec. A target region of X mRNA was chosen among 12 GUC-containing regions, because it is predicted not to be base-paired by a computer algorthm of RNA secondary structure prediction. Each target-binding, variable region was designed to contain 4 matched bases near the core sequence and 4 2-base wobbles (matched and mismatched) in the rest. Among 256 possible sequences 12 sequences have so far been identified to yield suppressed expression of the fused lacZ. The $\beta$-galactosidase activities were reduced at least 10-fold. Also corresponding polypeptedes were not produced as revealed by SDS-polyacrylamide gel electrophoresis.eng
dc.languageeng-
dc.publisher한국과학기술원-
dc.subject촉매활성 RNA.-
dc.titleSynthetic ribozyme suppressing HBV X gene expression = 합성된 리보핵산효소에 의한 HBV X 유전자 발현 억제-
dc.typeThesis(Master)-
dc.identifier.CNRN68313/325007-
dc.description.department한국과학기술원 : 생명과학과, -
dc.identifier.uid000911420-
dc.contributor.localauthorKang, Chang-Won-
dc.contributor.localauthor강창원-
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