Synthetic ribozyme suppressing HBV X gene expression = 합성된 리보핵산효소에 의한 HBV X 유전자 발현 억제

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Ribozymes are potentially very powerful agents for perturbing intracellular gene expression. Hammerhead structure ribozymes were designed to cleave the mRNA of hepatitis B virus X gene. Its cleavage was designed to result in the suppressed expression of lacZ fused to a 5$^\prime$462-bp fragment of X gene. The hammerhead ribozymes consist of a core region for cleavage activity and two variable regions for binding to a target RNA sequenec. A target region of X mRNA was chosen among 12 GUC-containing regions, because it is predicted not to be base-paired by a computer algorthm of RNA secondary structure prediction. Each target-binding, variable region was designed to contain 4 matched bases near the core sequence and 4 2-base wobbles (matched and mismatched) in the rest. Among 256 possible sequences 12 sequences have so far been identified to yield suppressed expression of the fused lacZ. The $\beta$-galactosidase activities were reduced at least 10-fold. Also corresponding polypeptedes were not produced as revealed by SDS-polyacrylamide gel electrophoresis.
Advisors
Kang, Chang-Wonresearcher강창원researcher
Description
한국과학기술원 : 생명과학과,
Publisher
한국과학기술원
Issue Date
1993
Identifier
68313/325007 / 000911420
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생명과학과, 1993.2, [ vi, 47 p. ]

Keywords

촉매활성 RNA.

URI
http://hdl.handle.net/10203/28401
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=68313&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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