DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Yang, Kyu-Hwan | - |
dc.contributor.advisor | 양규환 | - |
dc.contributor.author | Yang, Gi-Hyeok | - |
dc.contributor.author | 양기혁 | - |
dc.date.accessioned | 2011-12-12T08:59:27Z | - |
dc.date.available | 2011-12-12T08:59:27Z | - |
dc.date.issued | 1993 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=68312&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/28400 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 생명과학과, 1993.2, [ iv, 48 p. ] | - |
dc.description.abstract | The nucleotide sequences of the cloned Clostridium botulinum type B neurotoxin (BoNT/B) gene fragments were analyzed by dideoxy chain termination methods. The 2.5 Kb fragment coded for the C- terminus of L chain and H chain fragment. The nucleotide sequence for the BoNT/B fragment was determined, and derived amono acids sequence compared with those of botulinum neurotoxin type A (BoNT/A) and tetanus toxin (TeTx). It was found that there exist highly homologous regions and specially extremely conserved trp amino acid. 2.4 Kb HindIII fragment (containing N-terminus of H chain) and 1.9 Kb Xbal fragmant (containing not) were expressed in E. coli with pRSET, a plasmid vector containing the T7 promoter and a portion of the mbs gene. The fusion protein containing N-terminus of H chain were shown to react with, anti-BoNT/B monoclonal antibody BTBM4 recognizing H chain. This result suggests that the epitope of BTBM4 is located in N-terminus of H chain. With previous works (Davis and Hassen, 1988, Ham and Homsy, 1988). But the diffusivity values were larger by a factor of 5 since the self-sharpening effect is considered in this study. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.title | Nucleotide sequencing and expression of clostridium botulinum neurotoxin type B in escherichia coli | - |
dc.title.alternative | Clostridium botulinum Type B 신경독소의 염기서열 분석 및 escherichia coli에서의 발현 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 68312/325007 | - |
dc.description.department | 한국과학기술원 : 생명과학과, | - |
dc.identifier.uid | 000911351 | - |
dc.contributor.localauthor | Yang, Kyu-Hwan | - |
dc.contributor.localauthor | 양규환 | - |
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