Nucleotide sequencing and expression of clostridium botulinum neurotoxin type B in escherichia coli = Clostridium botulinum Type B 신경독소의 염기서열 분석 및 escherichia coli에서의 발현

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The nucleotide sequences of the cloned Clostridium botulinum type B neurotoxin (BoNT/B) gene fragments were analyzed by dideoxy chain termination methods. The 2.5 Kb fragment coded for the C- terminus of L chain and H chain fragment. The nucleotide sequence for the BoNT/B fragment was determined, and derived amono acids sequence compared with those of botulinum neurotoxin type A (BoNT/A) and tetanus toxin (TeTx). It was found that there exist highly homologous regions and specially extremely conserved trp amino acid. 2.4 Kb HindIII fragment (containing N-terminus of H chain) and 1.9 Kb Xbal fragmant (containing not) were expressed in E. coli with pRSET, a plasmid vector containing the T7 promoter and a portion of the mbs gene. The fusion protein containing N-terminus of H chain were shown to react with, anti-BoNT/B monoclonal antibody BTBM4 recognizing H chain. This result suggests that the epitope of BTBM4 is located in N-terminus of H chain. With previous works (Davis and Hassen, 1988, Ham and Homsy, 1988). But the diffusivity values were larger by a factor of 5 since the self-sharpening effect is considered in this study.
Advisors
Yang, Kyu-Hwan양규환
Description
한국과학기술원 : 생명과학과,
Publisher
한국과학기술원
Issue Date
1993
Identifier
68312/325007 / 000911351
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생명과학과, 1993.2, [ iv, 48 p. ]

URI
http://hdl.handle.net/10203/28400
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=68312&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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