Genetic transformation of tobacco plants with yeast acid phosphatase = 효모의 Acid phosphatase 유전자를 이용한 담배의 형질전환

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The pho5 gene of S. cerevisiae encodes the repressible acid phosphatase(rApase) which is regulated by the concentration of Pi in a medium. Introducting the pho5 gene into tobacco plants and allowing sebsequent expression under the control of CaMV 35S promotor, it is expected to make transgenic plants resistant to Pi deprivation in acidified soil. For this purpose, plasmid pBSW1 was constructed by the insertion of 1.5 kb DNA fraagment containing the entire open reading frame of pho5 gene into T-DNA region of pBKS1-1, a binary vector. Tobacco cells were transformed by co-incubating with A. tumefaciens cells carryng pBSW1. Transformed cells were regenerated into natural plants which have the T-DNA region of pBSW1 in their genome.
Advisors
Chung, Won-Ilresearcher정원일researcher
Description
한국과학기술원 : 생명과학과,
Publisher
한국과학기술원
Issue Date
1993
Identifier
68310/325007 / 000911252
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생명과학과, 1993.2, [ v, 49 p. ]

URI
http://hdl.handle.net/10203/28397
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=68310&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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