In Bacillus subtilis, many genes responsible for hyperproduction of extracellular enzymes have been reported and new ones are expected to be exist. prt R gene, for example, encodes a 60-amino acid polypeptide and can enhance the transcription of neutral and alkaline proteases and levansucrases. To clone prt R gene or other genes which has pleiotropic effect, a system was developed in which those genes could be cloned by simple Cm-selection. But this system was turned out to be unreliable because small portion of cells showed greatly enhanced Cm-resistancy. Using $^{32}P$-labelled oligonucleotide as a probe, one colony showed positive signal in colony hybridization. The recombinant plasmid contained a 1.65 kb fragment. When subcloned into B. subtilis MI112 cells, moderate change of production of extracellular proteases was detected on skim-milk plate. Further characterization should be needed.