DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Park, Hyun Gyu | - |
dc.contributor.advisor | 박현규 | - |
dc.contributor.author | Ju, Yong | - |
dc.date.accessioned | 2021-05-12T19:32:03Z | - |
dc.date.available | 2021-05-12T19:32:03Z | - |
dc.date.issued | 2018 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=887098&flag=dissertation | en_US |
dc.identifier.uri | http://hdl.handle.net/10203/283751 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 생명화학공학과, 2018.8,[iii, 24 p. :] | - |
dc.description.abstract | We herein developed a novel target RNA detection method called Nicking/Extension chain reaction System-Based isothermal Amplification (NESBA). In principle, the presence of target RNA induces formation of double-stranded (ds) DNA containing nicking sites and T7 promoter sequence which are utilized for nicking/extension chain reaction- and transcription-mediated isothermal amplification, respectively. As a result, RNA amplicons bind to the molecular beacons (MBs) leading to highly enhanced fluorescent signal. Based on this novel strategy, we successfully detected target genomic RNA (gRNA) derived from influenza A (H3N2) virus down to 3.6 ag/$\mu$L with high specificity over other gRNAs from different subtypes of influenza A virus. | - |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | RNA detection▼aIsothermal amplification▼aNicking/extension chain reaction▼aTranscription reaction▼aBiosensor | - |
dc.subject | RNA 검출▼a등온 증폭▼a절단 및 중합연쇄반응▼a전사 반응▼a바이오센서 | - |
dc.title | (A) novel target RNA detection utilizing nicking/extension chain reaction system-based isothermal amplification | - |
dc.title.alternative | 핵산의 절단 및 중합연쇄반응 시스템 기반 등온 증폭 기술을 이용한 새로운 표적 RNA 검출 기술 개발 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 325007 | - |
dc.description.department | 한국과학기술원 :생명화학공학과, | - |
dc.contributor.alternativeauthor | 주용 | - |
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