Maintenance of drug-metabolizing activities in primary cultures of adult rat hepatocytes = 일차 배양 간세포 내의 약물 대사능 유지에 관한 연구

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The maintenance effect of hexobarbital on drug-metabolizing enzyme activities were investigated in primary cultures of adult rat hepatocytes. Hexobarbital (lmM) was treated to culture media directly at the time of initial plating. The activities of drug-metabolizing enzymes, such as 7-ethoxycoumarin O-deethylase, aryl hydrocarbon hydroxylase, aminopyrine N-demethylase, and DMN demethylase were measured 24hr after culture. Hexobarbital increased the activities of drug-metabolizing enzymes in hormone-supplemented AB medium compared with WOBA-M medium. To elucidate these differences, the activities of 7-ethoxycoumarin O-deethylase of primary culture different culture conditions were examined. Hydrocortisone in AB medium significantly affected the maintenance effect of hexobarbital. The increase of drug-metabolizing enzyme activity by hexobarbital was proportional to the concentration of hydrocortisone supplemented in culture medium.
Advisors
Yang, Kyu-HwanYoo, Ook-Joon양규환유욱준
Description
한국과학기술원 : 생물공학과,
Publisher
한국과학기술원
Issue Date
1990
Identifier
67195/325007 / 000881493
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물공학과, 1990.2, [ vi, 48 p. ]

URI
http://hdl.handle.net/10203/28357
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=67195&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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