Continuous hydrolysis of olive oil by lipase immobilized on DEAE-sephadex = DEAE-sephadex 에 고정화된 리파제에 의한 올리브유의 연속적 가수분해에 대한 연구

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For the purpose of continuous hydrolysis of fats and lipids immobilized Candida rugosa lipase was used in packed bed reactor system. So as to broaden the substrate range up to solid fat and to enhance the productivity, isooctane was selected as a solvent. By the preliminary repeated batch experiment, DEAE-Sephadex was selected as immobilized matrix (data not shown). The immobilization efficiency was greater than 90\% for the loading capacity up to 20 mg protein/g-wet gel and the maximum loading could be obtained up to 40 mg/g-wet gel with the immobilization efficiency of 70\%. Apparent Km value and optimum pH was 8\% (v/v) olive oil in isooctane and 7. 0, respectively. In order to pack the gel in a column reactor, AOT was used and because of enzyme desorption by AOT, packing method was selected carefully. To increase the operational stability buffer, buffer-solvent ratio and the amount of loaded enzyme were varied. As the results of these experiments triethanol amino buffer was better than phosphate buffer, and less buffer flow rate and more enzyme-loading gel increased the operational stability. Under last condition the half life of immobilized enzyme was increased over 25 times.
Advisors
Rhee, Joon-Shick이준식
Description
한국과학기술원 : 생물공학과,
Publisher
한국과학기술원
Issue Date
1990
Identifier
67193/325007 / 000881459
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물공학과, 1990.2, [ viii, 58 p. ]

URI
http://hdl.handle.net/10203/28355
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=67193&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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