Development of high-sensitivity microfluidic chip calorimeter for cellular metabolic rate measurement세포대사량 측정을 위한 고민감도 미세유체 칩 열량계 개발

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Microfluidic chip calorimeter is a promising tool for cell-based assay because all living organisms and many related biological processes produce heat flux associated with the metabolic activity, an indicator of diseases. However, it has been challenging for chip calorimeters to provide both high sensitivity and accurate sample-manipulation capability. In the previous study, vacuum-insulated parylene microfluidics enabled the measurements of the heat generated from ~ nL scale samples with a few nW resolution. However, in the case of the chip calorimeter for measuring the metabolic rate of cells, an efficient measurement chamber for stable cells and drugs manipulation is required with improved sensitivity. Parylene microfluidic system provides excellent thermal insulation and physical strength to form a thin-film microfluidics. However conventional fabrication technique using sacrificial photoresist has problems of long fabrication time and contamination from residual photoresist inside channel. We developed a new molding and bonding method with nano-adhesive layers deposited by initiation chemical vapor deposition (iCVD) for the parylene microfluidics. In this study, the effective fabrication process was used for development of flexible microfluidics and cell and drug injection channel of chip calorimeter. Flexible thin-film parylene microfluidics was fabricated by completely detaching from the substrates and it was developed as a rollable system with micro-scale radius of curvature. The compact packed system and the tunable Dean flow mixer with reconfigurable 3D geometry of 2D microfluidic was demonstrated. A highly sensitive thin-film parylene microfluidic chip calorimeter with on-chip valve system capable of accurate sample-manipulation was developed. The chip calorimeter consisted of the vanadium oxide thermistors with high temperature coefficient of resistance of -3.2 %/K and the vacuum insulated parylene microfluidics. It provided excellent power resolution of a few nW with several hundred nL of sample. Using the fabricated chip calorimeter, we demonstrated the measurement of cellular metabolic rate by dissolving Hela cells.
Advisors
Lee, Wonheeresearcher이원희researcher
Description
한국과학기술원 :나노과학기술대학원,
Publisher
한국과학기술원
Issue Date
2018
Identifier
325007
Language
eng
Description

학위논문(박사) - 한국과학기술원 : 나노과학기술대학원, 2018.2,[vii, 89 p. :]

Keywords

Parylene microfluidics▼aMicrofluidic chip calorimeter▼aVacuum insulation▼aVanadium oxide▼aCell based assay; 페릴렌 채널▼a미세유체 칩 열량계▼a진공단열▼a바나듐옥사이드▼a세포 검정법

URI
http://hdl.handle.net/10203/283457
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=888330&flag=dissertation
Appears in Collection
NT-Theses_Ph.D.(박사논문)
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