Enhancement of polysaccharide-biopolymer productivity from methanol by methylobacterium organophilum = Methylobacterium organophilum에 의한 메탄올로부터 다당류계 생물고분자의 생산성 향상에 관한 연구

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In order to enhance the polysaccharide production from methanol by Methylobacterium organophilum, effects of addition of sugars and TCA-intermediates, and control of nitrogen fixation-favored conditions were investigated. The supplement of 4 g/l glucose and 0.01 M succinate to the fermentation medium increased the polysaccharide production by 2 times as compared with that obtained in only methanol containing medium as a organic substance. The other sugars showed no effects on cell growth and polysaccharide production, whereas malate had a slightly positive effects. By the intermittently feeding fed-batch culture using a DO-stat, effects of the dissolved oxygen level and the concentration of Mo$^{2+}$ and/or Fe$^{2+}$ were studied because these conditions might alter the nitrogen fixation activity of the cells. Cell growth was greatly repressed by oxygen-limitation but production yield(P/X) was not changed. When 6.4 mg/l sodium molybdate was added to the medium, cell growth and product yield were increased respectively 2.4 and 6.7 fold when compared with no Mo$^{2+}$ -containing control. The addition of FeSO4 increased both cell growth and Y$_{P/X}$ consistently with increasing concentration up to 10.8 mg/l. However, addition of FeSO4 into a medium containing excess sodium molybdate, resulted in reduced Y$_{P/X}$. Finally, by the addition of 6.4 mg/l Mo$^{2+}$ and 10.4 mg/l Fe$^{2+}$, 15 g/l cell mass and 8.5 g/l polysaccharide were obtained.
Advisors
Kim, Jung-Hoeresearcher김정회researcher
Description
한국과학기술원 : 생물공학과,
Publisher
한국과학기술원
Issue Date
1990
Identifier
67181/325007 / 000881196
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물공학과, 1990.2, [ v, 59 p. ]

URI
http://hdl.handle.net/10203/28343
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=67181&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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