Development of photoactivatable Flp recombinase (PA-Flp) and its application in the mouse brain빛으로 활성화되는 Flp 유전자 재조합 효소의 개발과 쥐 뇌에서의 응용

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dc.contributor.advisorWon Do Heo-
dc.contributor.advisor허원도-
dc.contributor.authorJung, Hyunjin-
dc.date.accessioned2021-05-11T19:40:46Z-
dc.date.available2021-05-11T19:40:46Z-
dc.date.issued2019-
dc.identifier.urihttp://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=886204&flag=dissertationen_US
dc.identifier.urihttp://hdl.handle.net/10203/283411-
dc.description학위논문(박사) - 한국과학기술원 : 생명과학과, 2019.2,[v, 82 p. :]-
dc.description.abstractSpatiotemporal control of gene expression or labeling is a valuable strategy for identifying functions of genes within complex neural circuits. Here, we developed a highly light-sensitive and efficient photoactivatable Flp recombinase (PA-Flp) that is suitable for genetic manipulation in vivo. The highly light-sensitive property of PA-Flp is ideal for activation in deep mouse brain regions by illumination with a noninvasive light-emitting diode. In addition, PA-Flp can be extended to the Cre-lox system through a viral vector-expressing Flp-dependent Cre-activation platform, thereby activating both Flp and Cre. Finally, we demonstrate that PA-Flp-dependent, Cre-mediated Cav3.1 silencing in the medial septum increases object-exploration behavior in mice. Thus, PA-Flp is a noninvasive, highly efficient, easy-to-use optogenetic module that offers a side-effect-free and expandable genetic-manipulation tool for neuroscience research.-
dc.languageeng-
dc.publisher한국과학기술원-
dc.subjectFlp recombinase▼aphotoactivatable Flp▼anoninvasive▼alight-inducible▼agenetic manipulation-
dc.subjectFlp 재조합효소▼a광활성 Flp▼a비침투성▼a빛 유도▼a유전자 조작-
dc.titleDevelopment of photoactivatable Flp recombinase (PA-Flp) and its application in the mouse brain-
dc.title.alternative빛으로 활성화되는 Flp 유전자 재조합 효소의 개발과 쥐 뇌에서의 응용-
dc.typeThesis(Ph.D)-
dc.identifier.CNRN325007-
dc.description.department한국과학기술원 :생명과학과,-
dc.contributor.alternativeauthor정현진-
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