DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Won Do Heo | - |
dc.contributor.advisor | 허원도 | - |
dc.contributor.author | Jung, Hyunjin | - |
dc.date.accessioned | 2021-05-11T19:40:46Z | - |
dc.date.available | 2021-05-11T19:40:46Z | - |
dc.date.issued | 2019 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=886204&flag=dissertation | en_US |
dc.identifier.uri | http://hdl.handle.net/10203/283411 | - |
dc.description | 학위논문(박사) - 한국과학기술원 : 생명과학과, 2019.2,[v, 82 p. :] | - |
dc.description.abstract | Spatiotemporal control of gene expression or labeling is a valuable strategy for identifying functions of genes within complex neural circuits. Here, we developed a highly light-sensitive and efficient photoactivatable Flp recombinase (PA-Flp) that is suitable for genetic manipulation in vivo. The highly light-sensitive property of PA-Flp is ideal for activation in deep mouse brain regions by illumination with a noninvasive light-emitting diode. In addition, PA-Flp can be extended to the Cre-lox system through a viral vector-expressing Flp-dependent Cre-activation platform, thereby activating both Flp and Cre. Finally, we demonstrate that PA-Flp-dependent, Cre-mediated Cav3.1 silencing in the medial septum increases object-exploration behavior in mice. Thus, PA-Flp is a noninvasive, highly efficient, easy-to-use optogenetic module that offers a side-effect-free and expandable genetic-manipulation tool for neuroscience research. | - |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | Flp recombinase▼aphotoactivatable Flp▼anoninvasive▼alight-inducible▼agenetic manipulation | - |
dc.subject | Flp 재조합효소▼a광활성 Flp▼a비침투성▼a빛 유도▼a유전자 조작 | - |
dc.title | Development of photoactivatable Flp recombinase (PA-Flp) and its application in the mouse brain | - |
dc.title.alternative | 빛으로 활성화되는 Flp 유전자 재조합 효소의 개발과 쥐 뇌에서의 응용 | - |
dc.type | Thesis(Ph.D) | - |
dc.identifier.CNRN | 325007 | - |
dc.description.department | 한국과학기술원 :생명과학과, | - |
dc.contributor.alternativeauthor | 정현진 | - |
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