DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Park, Chan-Kyu | - |
dc.contributor.advisor | 박찬규 | - |
dc.contributor.author | Koh, Woo-Suk | - |
dc.contributor.author | 고우석 | - |
dc.date.accessioned | 2011-12-12T08:58:30Z | - |
dc.date.available | 2011-12-12T08:58:30Z | - |
dc.date.issued | 1990 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=67172&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/28335 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 생물공학과, 1990.2, [ [iv], 49 p. ] | - |
dc.description.abstract | To study the regulation of drug metabolism, the expression of cytochrome P-450 isozymes and drug-metabolizing activities were investigated by using the primary cultures of adult rat hepatocytes. We used hexobarbital 1 mM concentration to maintain or induce the ability of drug metabolism of cultured hepatocytes. Addition of hexobarbital to the culture medium showed time-dependent increases of the cytochrome P-450 associated monooxygenases, such as ethoxycoumarin O-deethylase, aryl hydrocarbon hydroxylase, aniline 4-hydroxylase, and ethoxyresolufin O-decthylase. These increments of enzyme activities were abolished by addition of cyclocheximide (1.0 uM). We also could obtain the increase of the 3-methylcholanthrene-inducible cytochrome P-450 isozyme and phenobarbital inducible P-450 isozyme in radioimmunocassay. In conclusion, it seems that hexobarbital may induce the cytochrome P450 and monooxygenase activities in culture and these induction are dependent on protein synthesis. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.title | Expression of drug-metabolizing activities by hexobarbital in primary cultures of adult rat hepatocytes | - |
dc.title.alternative | Hexobarbital에 의한 일차 배양 간세포의 약물 대사능 발현에 관한 연구 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 67172/325007 | - |
dc.description.department | 한국과학기술원 : 생물공학과, | - |
dc.identifier.uid | 000881014 | - |
dc.contributor.localauthor | Park, Chan-Kyu | - |
dc.contributor.localauthor | 박찬규 | - |
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