Expression of drug-metabolizing activities by hexobarbital in primary cultures of adult rat hepatocytes = Hexobarbital에 의한 일차 배양 간세포의 약물 대사능 발현에 관한 연구

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dc.contributor.advisorPark, Chan-Kyu-
dc.contributor.advisor박찬규-
dc.contributor.authorKoh, Woo-Suk-
dc.contributor.author고우석-
dc.date.accessioned2011-12-12T08:58:30Z-
dc.date.available2011-12-12T08:58:30Z-
dc.date.issued1990-
dc.identifier.urihttp://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=67172&flag=dissertation-
dc.identifier.urihttp://hdl.handle.net/10203/28335-
dc.description학위논문(석사) - 한국과학기술원 : 생물공학과, 1990.2, [ [iv], 49 p. ]-
dc.description.abstractTo study the regulation of drug metabolism, the expression of cytochrome P-450 isozymes and drug-metabolizing activities were investigated by using the primary cultures of adult rat hepatocytes. We used hexobarbital 1 mM concentration to maintain or induce the ability of drug metabolism of cultured hepatocytes. Addition of hexobarbital to the culture medium showed time-dependent increases of the cytochrome P-450 associated monooxygenases, such as ethoxycoumarin O-deethylase, aryl hydrocarbon hydroxylase, aniline 4-hydroxylase, and ethoxyresolufin O-decthylase. These increments of enzyme activities were abolished by addition of cyclocheximide (1.0 uM). We also could obtain the increase of the 3-methylcholanthrene-inducible cytochrome P-450 isozyme and phenobarbital inducible P-450 isozyme in radioimmunocassay. In conclusion, it seems that hexobarbital may induce the cytochrome P450 and monooxygenase activities in culture and these induction are dependent on protein synthesis.eng
dc.languageeng-
dc.publisher한국과학기술원-
dc.titleExpression of drug-metabolizing activities by hexobarbital in primary cultures of adult rat hepatocytes = Hexobarbital에 의한 일차 배양 간세포의 약물 대사능 발현에 관한 연구-
dc.typeThesis(Master)-
dc.identifier.CNRN67172/325007-
dc.description.department한국과학기술원 : 생물공학과, -
dc.identifier.uid000881014-
dc.contributor.localauthorPark, Chan-Kyu-
dc.contributor.localauthor박찬규-
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