Expression of drug-metabolizing activities by hexobarbital in primary cultures of adult rat hepatocytes = Hexobarbital에 의한 일차 배양 간세포의 약물 대사능 발현에 관한 연구

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To study the regulation of drug metabolism, the expression of cytochrome P-450 isozymes and drug-metabolizing activities were investigated by using the primary cultures of adult rat hepatocytes. We used hexobarbital 1 mM concentration to maintain or induce the ability of drug metabolism of cultured hepatocytes. Addition of hexobarbital to the culture medium showed time-dependent increases of the cytochrome P-450 associated monooxygenases, such as ethoxycoumarin O-deethylase, aryl hydrocarbon hydroxylase, aniline 4-hydroxylase, and ethoxyresolufin O-decthylase. These increments of enzyme activities were abolished by addition of cyclocheximide (1.0 uM). We also could obtain the increase of the 3-methylcholanthrene-inducible cytochrome P-450 isozyme and phenobarbital inducible P-450 isozyme in radioimmunocassay. In conclusion, it seems that hexobarbital may induce the cytochrome P450 and monooxygenase activities in culture and these induction are dependent on protein synthesis.
Advisors
Park, Chan-Kyuresearcher박찬규researcher
Description
한국과학기술원 : 생물공학과,
Publisher
한국과학기술원
Issue Date
1990
Identifier
67172/325007 / 000881014
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물공학과, 1990.2, [ [iv], 49 p. ]

URI
http://hdl.handle.net/10203/28335
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=67172&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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