DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Kim, Injune | - |
dc.contributor.advisor | 김인준 | - |
dc.contributor.advisor | Cho, You Sook | - |
dc.contributor.advisor | 조유숙 | - |
dc.contributor.author | Ha, Eun Hee | - |
dc.date.accessioned | 2021-05-11T19:38:08Z | - |
dc.date.available | 2021-05-11T19:38:08Z | - |
dc.date.issued | 2019 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=871436&flag=dissertation | en_US |
dc.identifier.uri | http://hdl.handle.net/10203/283266 | - |
dc.description | 학위논문(박사) - 한국과학기술원 : 의과학학제전공, 2019.8,[viii, 93 p. :] | - |
dc.description.abstract | Asthma is a heterogeneous disease with chronic inflammation and remodeling of airway. Sox17 is regarded as a transcription factor of vascular network regulating various pro-angiogenic target genes. Recently, IL-33, a critical cytokine linking to recruitment and activation of ILC2 prior to Th2 immune responses, was reported induce up-regulation of Sox17 transcription. However, the role of Sox17 related with IL-33 in the development of allergic airway inflammation was not clearly evaluated. We established conventional mouse model of asthma in WT and Sox17 endothelial conditional knock-out mice, and evaluated airway inflammation in BAL fluid and lung tissues. We sorted endothelial cells from these mice and evaluated various chemical mediators by qRT-PCR. To investigate underlying mechanism in endothelial cells, Sox17 was silenced or over-expressed in IL-33 activated HUVECs and HPMECs then evaluated various cytokines and chemokines. In the airway of asthmatic mice, Sox17 expression was significantly enhanced, and absence of Sox17 was resulted in down-regulation of Th2 airway inflammation. Among inflammatory cells, inflammatory dendritic cells and monocytes were decreased, and these were accompanied by down-regulation of cytokines and adhesion molecules in sorted endothelial cells from lung tissues. IL-33 stimulated endothelial cells showed positive correlation between Sox17 expression and chemokine secretion after knock-down or over-expression of Sox17. These results demonstrated that Sox17 is involved with the development of allergic airway inflammation through IL-33 associated activation of endothelial cells. | - |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | Sox17▼aIL-33▼aasthma▼aendothelial activation▼aallergic airway inflammation | - |
dc.subject | 삭스 17▼a아이엘-13▼a천식▼a내피세포 활성화▼a알레르기 기도 염증 | - |
dc.title | Role of endothelial Sox17 in allergic airway inflammation | - |
dc.title.alternative | 알레르기 염증반응에서 Sox17의 역할 | - |
dc.type | Thesis(Ph.D) | - |
dc.identifier.CNRN | 325007 | - |
dc.description.department | 한국과학기술원 :의과학학제전공, | - |
dc.contributor.alternativeauthor | 하은희 | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.