Construction of a hybrid T7/SP6 RNA polymerase gene = T7과 SP6 RNA 중합효소의 혼성 유전자 제조

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To clone the phage SP6 RNA polymerase gene the Hind III digestion fragment containing the gene was first mapped with restriction endonucleases Bst XI, Dra I and Kpn I. The Kpn I cleaves the structural gene into two parts. The downstream three fifths of the gene from Kpn I site to Bst XI site was successfully cloned into the plasmid pUC19 and partially sequenced. Using the unique and common Hpa I site of the SP6 and T7 RNA polymerase genes, has been constructed a hybrid T7/SP6 RNA polymerase gene that contains the upstream two thirds of T7 gene and the downstream one third of SP6 gene, from the cloned SP6 and T7 RNA polymerase genes.
Advisors
Kang, Chang-Wonresearcher강창원researcher
Description
한국과학기술원 : 생물공학과,
Publisher
한국과학기술원
Issue Date
1989
Identifier
66655/325007 / 000871193
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물공학과, 1989.2, [ v, 53 p. ]

URI
http://hdl.handle.net/10203/28318
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=66655&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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