Transcription terminations of phage T7 and SP6 RNA polymerases = 파아지 SP6 와 T7 RNA 중합효소의 전사종결

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The in vitro transcription termination efficiencies of a phage T7 terminator T$\varphi$ for the T7 and SP6 RNA polymerases were studied. Amounts of transcription terminated by T$\varphi$ were compared with amounts of read-through transcripts run off at a restriction site. For quantitative analysis the transcripts were labeled with [a-32P] rNTP in invitro transcription reaction. Transcription of T7 RNA polymerase terminates with efficiency of 38\% by the T$\varphi$ in the pGEM3Z, but with increased efficiency in the pET-3. Interestingly, the T$\varphi$ shows 81\% termination efficiency for the SP6 RNA ploymerase. Now this becomes the first strong terminator discovered for the SP6 RNA polymerase. This also suggests that both RNA polymerases might share a similar mechanism of transcription termination.
Advisors
Kang, Chang-Wonresearcher강창원researcher
Description
한국과학기술원 : 생물공학과,
Publisher
한국과학기술원
Issue Date
1989
Identifier
66647/325007 / 000871072
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물공학과, 1989.2, [ v, 45 p. ]

URI
http://hdl.handle.net/10203/28310
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=66647&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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