Effect of lysine residue modification on stability and activity of candida rugosa lipase = 라이신 잔기의 화학적 변형이 candida rugosa 리파제의 활성과 안정성에 미치는 영향

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To improve the thermostability and organic solvent stability of the lipase, chemical modifications of the enzyme were performed. Lysine residues of Candida rugosa lipase were acetylated and succinylated, and activity and stability of these modified enzymes were examined in reversed micelle system. UV difference adn flurosence spectroscopic studies were also performed to et some information about structural changes of the modifiedenzyme. Both actylation and succinylation resulted in increase of substrate affinity, thermal stability and organic solvent stability of the enzyme. And there was no significant structrual changes by the modification. From the results of the modification, at least one of the cysteine, histidine and tyrosine residue was involved in active site of the enzyme and the histidine residue is most probable. Lysine residue was not involved in active site of the enzyme.
Advisors
Rhee, Joon-Shick이준식
Description
한국과학기술원 : 생물공학과,
Publisher
한국과학기술원
Issue Date
1988
Identifier
66144/325007 / 000861240
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물공학과, 1988, [ vii, 65 p. ]

Keywords

단백질 화학 변형.

URI
http://hdl.handle.net/10203/28302
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=66144&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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