Extracellular polysaccharide production by methylobacterium organophilum NCIB 11278 from methanol = Methanol로부터 methylobacterium organophilum NCIB 11278 을 이용한 다당류의 생산

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Some methylotrophs were studied with their ability of poly-$\beta$-hydroxybutyrate production and extracellular polysaccharide production from methanol as a sole source of carbon. Methylobacterium organophilum NCIB 11278, a facultative methylotroph, originally isolated as a methane-utilizing bacterium, not only accumulates the poly- v$\beta$ -hydroxybutyrate, but also produces the extracellular polysaccharide on a minimum synthetic medium. For the efficient production of the polysaccharide from Methylobacterium organophilum NCIB 11278, the effect of the medium components and culture conditions were studied in batch culture. The optimal concentrations of medium components for maximum polysaccharide production were as follows; Methanol, 0.5\% (v/v); $(NH_4)_2SO_4$, 0.3 g /l; $Na_2HPO_4$, 1.06 g/l; $KH_2PO_4$, 0.65 g/l; $MgSO_4$, 0.45 g /l; and trace elements. The optimal pH and temperature for polysaccharide production were 6-7 and $30\,^\circ\!C$, respectively. This organism grew on 0.25\% (v/v) methanol with a maximum specific growth rate of 0.28 $hr^{-1}$. Methanol concentration higher than 4.0\% (v/v) was toxic. The maximum polysaccharide productivity (P/X) was obtained 0.42 g-polysaccharide/ g-DCW with 0.5\% methanol in batch culture. The substrate (methanol) inhibition and toxicity in polysaccharide production were recovered by constant fed-batch culture which was carried out by a fixed feeding rate (1.0 ml/min) with methanol containing media. The production of polysaccharide was stimulated by deficiency of nutrients except carbon source. By constant fed-batch culture, the polysaccharide was produced to 0.95 g together with 1.0 g of dry cells, per liter of the culture broth. The maximal productivity on methanol was enhanced to more than twice times by constant fed-batch culture as compared with the batch culture. And the optimal C/N molar ratio of feeding liquid is 100.
Advisors
Kim, Jung-HoeresearcherRhee, Joon-Shickresearcher김정회researcher이준식researcher
Description
한국과학기술원 : 생물공학과,
Publisher
한국과학기술원
Issue Date
1988
Identifier
66142/325007 / 000861446
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물공학과, 1988.2, [ vi, 55 p. ]

URI
http://hdl.handle.net/10203/28300
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=66142&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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