Isolation of aniline dissimilating bacteria and characterization of aniline biodegradation in pseudomonas testosteroni 6F1 and pseudomonas acidovorans 4A1 = Aniline이용 균주의 분리 및 분리 균주들에 의한 aniline이용 특성

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The microorganisms grown on a minimal medium with aniline as a sole source of carbon, nitrogen, and energy were isolated, identified, adn characterized. Pseudomonas acidovorans strain 4Al harbored a plasmid of ca. 230 kilobases, shown to be involved in aniline metabolism. The substrate specificity of aniline-dioxygenase from Pseudomonas testosteroni strain 6Fl was found to be restricted to native form of aniline, indicating that it did not act on aniline derivatives or cationic state of aniline. In addition, this enzyme might not be coordinated with other enzymes of the -keto-adipate pathway. Utilization of aniline was promoted by the addition of other utilizable crbon sources to the bacterial cells of P. testosteroni strain 6Fl in lag phase and in starved condition. It was shown that the NADH level in the cells related to the aniline uptake rate.
Advisors
Kho, Yung-Hee고영희
Description
한국과학기술원 : 생물공학과,
Publisher
한국과학기술원
Issue Date
1988
Identifier
66138/325007 / 000861509
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물공학과, 1988.2, [ ii, 57 p. ]

URI
http://hdl.handle.net/10203/28296
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=66138&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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