Cloning of bacillus gene possessing β-fructofuranosidase activity in escherichia coli = 고초균의 β-fructofuranosidase 활성을 가지는 유전자의 escherichia coli 에로의 클로닝

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A inulase producing spore-former had been isolated, identified as a Bacillus subtilis and used as gene dono. The purified genomic DNA from Bacillus subtilis was digested with PstI and eluted by electrophoresis to four fraction according to the size. Fractions 1, 2, 3, 4 corresponding average 13.5 kb, 3 kb, 1.5 kb, 0.6 kb in size. Fractions 1 and 2 were ligated with a vehicle, pUC9 which was digested with PstI adn dephosphorylated with CIP. The ligated DNA was transformed into competent E. coli JM 103 cells and spreaded on the tetrazolium plates. After incubation at 37$^\circ$C for 24 hours, one transformant that formed large and red color colony was selected adn assayed for inulase activity. That transformant showed 0.8 times less inulase activity than the original Bacillus subtilis JU 70. The insert size of the plasmid carrying the inulase gene was 2.1 kb. The restriction map of recombinant plasmid was constructed using restiriction enzymes AvaII, EcoRI, HindIII, PstI, SacI et al.
Advisors
Byun, Si-MyungPack, Moo-Young변시명박무영
Description
한국과학기술원 : 생물공학과,
Publisher
한국과학기술원
Issue Date
1988
Identifier
66134/325007 / 000861384
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물공학과, 1988.2, [ vi, 57 p. ]

URI
http://hdl.handle.net/10203/28292
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=66134&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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