In vitro synthesis of 5 S rRNA using phage SP6 RNA polymerase = 파아지 SP6 RNA 중합효소를 이용한 5 S rRNA 의 합성

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The in vitro transcripts of phage SP6 RNA polymerase usually contain extraneous 5`` and 3`` plasmid sequences in addition to the cloned gene sequence. Template plasmids were constructed to eliminate these extraneous sequences. The 5 S RNA gene of Xenopus borealis somatic cell was inserted at the transcription initiation site (BamHI site) of PCKSP6 so that the transcription starts from the 5`` end of the gene. The restriction enzyme DraI site was inserted at the 3`` end of 5 S RNA structrual gene by oligonucleotide-directed site-specific mutagenesis. Now, when the recombinant DNA is digested with DraI, the resulting in vitro run-off transcripts of the linearized template using phage SP6 RNA polymerase contain the authentic 5`` and 3``ends. Thus, the large quantity of authentic 5 S RNA and any mutant RNAs, which could easily be radioactively labeled, can be produced for structure-function relationship studies, RNA folding studies, and other biophysical studies that have been limited by sample quantities.
Advisors
Kang, Chang-Wonresearcher강창원researcher
Description
한국과학기술원 : 생물공학과,
Publisher
한국과학기술원
Issue Date
1988
Identifier
66131/325007 / 000861326
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물공학과, 1988.2, [ v, 40 p. ]

URI
http://hdl.handle.net/10203/28289
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=66131&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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