Molecular cloning of subtilisin gene = 서브틸리신 유전자의 Molecular cloning

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The purified genomic DNA from Bacillus natto was digested with PstI and eluted by electrophoresis to 2 fractions according to the size. Fraction 1, 2 corresponding 4-20kb, 2-4kb were ligated with a vehicle, pUC9 which was digested with PstI and dephosphorylatedwith CIP. The ligated DNA was transformed into competent Escherichia coli JM83 cells. Around 10,000 colonies were appeared on the selective media containing ampicillin. These transforemdcolonies were tested which hybridized to the probe. The No. FII-2504 colony showed positive spot. The insert size of the plasmid carrying the protease gene was 2.2kb and designated as pSEl. The restriction maps of pSEl was constructed by digesting with Pstl, ScaI, EcoRI, HindIII, respectively. The cell harbouring pSEl showed 1.3-fold high protease activity than control Escherichia coli JM83.
Advisors
Byun, Si-MyungPack, Moo-Young변시명박무영
Description
한국과학기술원 : 생물공학과,
Publisher
한국과학기술원
Issue Date
1988
Identifier
66130/325007 / 000851184
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물공학과, 1988.2, [ vii, 51 p. ]

URI
http://hdl.handle.net/10203/28288
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=66130&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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