Transcription initiation site of phage SP6 RNA polymerase = 파아지 SP6 RNA 중합효소의 전사개시위치

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The transcription initiation sites of the phage SP6 initiation sequence mutants containing deletions around the wild-type initiation site were determined. It could be possible to obtain sequencing ladders in high-resolution polyacrylamide-urea gel electrophoresis, using the phage SP6 RNA polymerase under the in vitro transcription conditions where the limiting concentration of a ribonucleotide causes the polymerase to pause long enough and terminate at the positions of the nucleotide. Precise sizing of the transcripts produced by this abortive elongation determined the initiation site of each mutant. When the wild-type position +1 G is changed to C, it still starts at the C. Also the wild-type position +1 G is changed to A, it still starts at the A. But, the mutant containing CCC sequence from -1 to +2 starts transcription at the C at both the wild-type position -1 and +1 with equal frequency.
Advisors
Kang, Chang-Wonresearcher강창원researcher
Description
한국과학기술원 : 생물공학과,
Publisher
한국과학기술원
Issue Date
1988
Identifier
66124/325007 / 000861123
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물공학과, 1988.2, [ vi, 51 p. ]

URI
http://hdl.handle.net/10203/28282
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=66124&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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