DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Yang, Kyu-Hwan | - |
dc.contributor.advisor | 양규환 | - |
dc.contributor.author | Ha, Jong-Ryul | - |
dc.contributor.author | 하종열 | - |
dc.date.accessioned | 2011-12-12T08:57:32Z | - |
dc.date.available | 2011-12-12T08:57:32Z | - |
dc.date.issued | 1987 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=65594&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/28269 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 생물공학과, 1987.2, [ iv, 49 p. ] | - |
dc.description.abstract | The inducibility of cytochrome P-450 associated monooxygenase was studied in primary cultures of ault rat hepatocytes. Rats were pretreated with phenobarbital (PB) or 3-methylcholanthrene (3-MC) and hepatocytes were isolated from PB-, 3-MC-treated, and untreated rats by collagenase perfusion technique. At the initial time of isolation, 3-MC enhanced 7-ethoxycoumarine-0-deethylase, Aryl hydrocarbon hydroxylase and biphenyl-4-hydroxylase by a factor of 12,60 and 2.5, except for aminopyrine-N-demethylase, respectively. The activities of enzymes were maintained higher level until 72 hrs when compared to untreated groups. Phenobarbital-pretreatment showed similar inducibility, but the magnitutes in induced enzymes level were relatively low compared to those by 3-MC. To conform the enhanced effects of metabolizing capability, unscheduled DNA synthesis (UDS) by Aflatoxin $B_1 (AF-B_1)$ was measured 24 hr after isolation of hepatocytes. UDS induced by $AF-B_1$ was slightly increased in PB-pretreated hepatocytes while decreased at low dose of $AF-B_1$ in 3-MC-pretreated hepatocytes compared to that in naive hepatocytes. These results suggest that the metabolizing capability of primary cultures of hepatocytes is increased by pretreatment of rats with PB or 3-MC. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.title | Measurement of unscheduled DNA synthesis by aflatoxin B1in primary culture of hepatocytes from rats pretreated with phenobarbital and 3-methylcholanthrene in vivo | - |
dc.title.alternative | 일차 배양 간 세포내 에서 aflatoxin B1 에 의한 비주기성 DNA 합성의 측정 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 65594/325007 | - |
dc.description.department | 한국과학기술원 : 생물공학과, | - |
dc.identifier.uid | 000851436 | - |
dc.contributor.localauthor | Yang, Kyu-Hwan | - |
dc.contributor.localauthor | 양규환 | - |
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