DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Yang, Kyu-Hwan | - |
dc.contributor.advisor | 양규환 | - |
dc.contributor.author | Park, Seong-Kyu | - |
dc.contributor.author | 박성규 | - |
dc.date.accessioned | 2011-12-12T08:57:21Z | - |
dc.date.available | 2011-12-12T08:57:21Z | - |
dc.date.issued | 1987 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=65582&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/28257 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 생물공학과, 1987.2, [ iv, 49 p. ] | - |
dc.description.abstract | In vitro induction of cytochrome P-450 associated monooxygenases by pehenobarbiatal(PB) and 3-methylcholanthrene(3-MC) was investigated in primary cultures of adult rat hepatocytes. 3-MC and PB were treated 24 hr often plating of hepatocytes and four monooxygenases activities were measured. 7-Ethoxycoumarin 0-deethylase and aryl hydrocarbon hydroxylase activities were increased to higher level than initial value by MC but, by PB, were increased to similar level of initial value. Biphenyl 4-hydroxylase and aminopyrine N-demethylase activities did not increased to initial level but maintenance effects could be seen. Induction pattern of PB and 3-MC was characteric. DNA repair synthesis was measured by determining $^3H$-thymidine incorporation into DNA for aflatoxin $B_1$ treated hepatocytes. Unscheduled DNA synthesis experiments were performed for untreated, PB and 3-MC treated hepatocytes. At the lower concentration of $AFB_1$, the same extent of unscheduled DNA synthesis was shown in untreated and inducer treated hepatocytes. However, at the higher concentration of $AFB_1$, in vitro administration of inducers enhanced unscheduled DNA synthesis induced by $AFB_1$. These results showed that this system may have application in the investigation of hepartocarcinogenesis by chemicals in vitro. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.title | In vitro induction of cytochrome P-450 associated monooxygenase activities in primary cultures of adult rat hepatocytes | - |
dc.title.alternative | 일차 배양 간세포에서의 약물대사 효소 활성의 항진 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 65582/325007 | - |
dc.description.department | 한국과학기술원 : 생물공학과, | - |
dc.identifier.uid | 000851135 | - |
dc.contributor.localauthor | Yang, Kyu-Hwan | - |
dc.contributor.localauthor | 양규환 | - |
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