(The) relationship between the binding of cytochrome c to vesicles and the cytochrome c induced fusion of vesicles = Cytochrome C 의 인지질막에 대한 결합과 융합 사이의 관계에 대한 연구

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The binding of cytochrome c to phospholipid vesicles was studied by the ultracentrifugation method as a function of pH and ionic strength in order to correlate the binding properties with membrane fusogenic properties of this protein. The pH dependent binding profile of the cytochrome c is complicated showing binding maxima around pH3 and pH9. Appreciable binding persistes even at high ionic strength and the binding reaction is partially irreversible indicating the involvement of hydrophobic interaction. The cytochrome c induced fusion of phospholipid vesicles, as observed by the intermixing of vesicles contents, also shows a complicated pattern. Above pH7, there is a fusion maximum at around 9. Below pH7, however, the extent of fusion increases steadily with decreasing pH. The relationship between the binding of cytochrome c to vesicles and the cytochrome c induced fusion of vesicles is discussed in terms of hydrophobic interaction between this protein and vesicles.
Advisors
Kim, Hyoung-Man김형만
Description
한국과학기술원 : 생물공학과,
Publisher
한국과학기술원
Issue Date
1986
Identifier
65051/325007 / 000841226
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물공학과, 1986.2, [ iii, 61 p. ]

URI
http://hdl.handle.net/10203/28242
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=65051&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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