DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Kim, Jung-Hoe | - |
dc.contributor.advisor | 김정회 | - |
dc.contributor.author | Nam, Soo-Wan | - |
dc.contributor.author | 남수완 | - |
dc.date.accessioned | 2011-12-12T08:56:56Z | - |
dc.date.available | 2011-12-12T08:56:56Z | - |
dc.date.issued | 1986 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=65038&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/28229 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 생물공학과, 1986.2, [ vii, 53 p. ] | - |
dc.description.abstract | For the analysis of the dynamic behaviour of population and plasmid instability in recombinant DNA cell fermentation system, a chemostat culture was performed using a mutant of E. coli \3110 Δtrp LD $rp R^{ts}$ $tna^-$/P CRT 185 harboring a whole trp-operon. The specific growth rates of plasmid-harboring and plasmid-free host cells were $0.48 hr^{-1}$ and $0.59 hr^{-1}$ at the derepressed condition (42℃), respectively. The relationship between the specific production rate of tryptophan, qp, and the specific growth rate, μ, was parabolic; at $μ = 0.25 hr^{-1}$, qp was about 3.8mg/g-cell hr, and at $μ = 0.075 hr^{-1}$ qp was about 10.3mg/g-cell hr. The plasmid DNA content per g-cell was observed to increased from 0.2 to 0.5 mg per g-cell as dilution rate decreased from 0.425 to $0.075 hr^{-1}$. At dilution rate of $0.25 hr^{-1}$ and repressed condition (37℃), plasmid stability is stably maintained for 225 hr. When tryptophan was added, however, plasmid stability was oscillated. At derepressed condition (42℃), the number of plasmid harboring cells decreased due to the plasmid instability as culture time elapsed, meanwhile the number of plasmid-free cells was increased. When tryptophan concentration in culture broth was reached a zero level, plasmid free cells could not grow. The cells, consequently, was washed out. On the contrary, when tryptophan ($1×10^{-3}M$ was continuously added to the culture medium, culture broth was dominant with plasmid-free cells after 200 hrs and the cell concentration reached at steady state. A two-stage continuous culture coupled with the cell growth in first stage at 37℃and the gene expression in second stage at 42℃, enhanced significantly the plasmid stability (above 96%) and maintained almost constantly the specific production rate of tryptophan for more than 500 hrs. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.title | (A) study on the dynamic behaviours of a recombinant E. coli cell and stable maintenance of a recombinant plasmid in chemostat culture | - |
dc.title.alternative | 연속 배양에서 재조합 E. coli 의 동적 양상과 재조합 plasmid 의 안정한 유지법에 관한 연구 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 65038/325007 | - |
dc.description.department | 한국과학기술원 : 생물공학과, | - |
dc.identifier.uid | 000841088 | - |
dc.contributor.localauthor | Kim, Jung-Hoe | - |
dc.contributor.localauthor | 김정회 | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.