Optimizing illumination in three-dimensional deconvolution microscopy for accurate refractive index tomography

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In light transmission microscopy, axial scanning does not directly provide tomographic reconstruction of specimen. Phase deconvolution microscopy can convert a raw intensity image stack into a refractive index tomogram, the intrinsic sample contrast which can be exploited for quantitative morphological analysis. However, this technique is limited by reconstruction artifacts due to unoptimized optical conditions, which leads to a sparse and non-uniform optical transfer function. Here, we propose an optimization method based on simulated annealing to systematically obtain optimal illumination schemes that enable artifact-free deconvolution. The proposed method showed precise tomographic reconstruction of unlabeled biological samples. (C) 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
Publisher
OPTICAL SOC AMER
Issue Date
2021-03
Language
English
Article Type
Article
Citation

OPTICS EXPRESS, v.29, no.5, pp.6293 - 6301

ISSN
1094-4087
DOI
10.1364/OE.412510
URI
http://hdl.handle.net/10203/282200
Appears in Collection
PH-Journal Papers(저널논문)
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