DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Han, Moon-Hi | - |
dc.contributor.advisor | 한문희 | - |
dc.contributor.author | Ha, Hyun-Jung | - |
dc.contributor.author | 하현정 | - |
dc.date.accessioned | 2011-12-12T08:56:47Z | - |
dc.date.available | 2011-12-12T08:56:47Z | - |
dc.date.issued | 1985 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=64509&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/28218 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 생물공학과, 1985.2, [ v, 59 p. ] | - |
dc.description.abstract | The conditions for the production of a T cell growth factor (TCGF or IL-2) capable of maintaining the long-term growth of activated lymphocytes and present in the supernatants of mouse stimulated EL-4 thymoma cells were investigated. IL-2 was induced in spleen cells and various T cell lines stimulated with T cell lectins and/or PMA (phorbol-12-myristate-13-acetate). The kinetics of IL-2 production by EL-4 cells were highly dependent on the cellular concentrations, concentration of serum, and inducers in the culture media, and especially incubation time. The maximum activity of IL-2 appeared in the early phase with high cell concentration and in the late phase with low concentration of cells. The kinetic study of IL-2 production in EL-4 cells demonstrated the maximum activity of IL-2 at 24hr incubation and the subsequent dramatic decrease was observed in the late phase of the culture. The rate of IL-2 production also appeared in the same pattern. The arrest of IL-2 activity in the late phase seemed to be due to plausible types of regulatory mechanism ; the depletion of some essential components in the induction system, feedback inhibition by IL-2 formed in the early phase of the culture, and IL-2 absorption by EL-4 cells in the different phase of the culture. The possible regulatory factors will be discused. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.title | (The) optimal condition and possible regulatory factor for IL-2 production in mouse thymoma EL-4 cell | - |
dc.title.alternative | 쥐의 흉선종양 세포인 EL-4 세포에서 IL-2(interleukin-2) 생성최적화 및 조절요인에 대한 연구 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 64509/325007 | - |
dc.description.department | 한국과학기술원 : 생물공학과, | - |
dc.identifier.uid | 000831657 | - |
dc.contributor.localauthor | Han, Moon-Hi | - |
dc.contributor.localauthor | 한문희 | - |
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