Indirect enzyme-linked immunosorbent assay for detection of clostridium botulinum type F toxin in microtiter plate = Microplate 에서의 closstridium botulinum type F toxin 검출의 면역학적 효소방법에 관한 연구

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The enzyme-linked immunosorbent assay using the "indirect" method in microscale was applied to determine the type F toxin of Clostridium botulinum. The absorbance profiles of four different combinations of anti-botulinum serum and conjugate were compared each other. Three different concentrations of Tween 20 in diluent (0.05, 0.1, and 0.2\%) were examined to search the optimal condition. The change of concentration of Tween 20 in diluent did not affect significantly the absorbance profile beside the combination of horse anti-botulinum serum as bridging antibody and rabbit conjugate. The minimum dectectable dose of type F toxin by "indirect" ELISA was found to be 10 mouse ip LD$_{50}$ and the quantitative assay was also possible. For the quantitative assay in range of $10^2$ to $10^4$ mouse ip LD 50 dose of type F toxin, two combinations in which mouse antiserum rabbit antiserum was used as bridging antibody were suitable for "indirect" ELISA. But the combination which showed the linear increment of absorbance over full range tested (10 to $10^4$ mouse ip LD$_{50}$), was that of horse anti-botulinum serum as bridging antibody.
Advisors
Yang, Kyu-HwanPack, Moo-Young양규환박무영
Description
한국과학기술원 : 생물공학과,
Publisher
한국과학기술원
Issue Date
1985
Identifier
64505/325007 / 000831305
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물공학과, 1985.2, [ [iv], 42 p. ]

URI
http://hdl.handle.net/10203/28214
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=64505&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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