(A) study on purification of rifamycin B oxidase by affinity chromatography and its biochemical properties

Abstract

A study on purification of rifamycin B oxidase by affinity chromatography and its biochemica properties. Purification of rifamycin B oxidase from a microbial strain, Humicola spp. ATCC 20620, which is known to catalyze the oxidative reaction of rifamycin B to rifamycin O, was achieved using affinity chromatography as a single purification step. The preparation technique of affinity gels in affinity chromatography involves (a) Covalent attachment of ligands to matrix gels through amino, carboxyl, phenolic, alcoholic hydroxyl, or keto groups. (b) Changing the length of ligands attached to the gel matrix backbone. An optimal condition for the preparation of affinity chromatography column was investigated in this work. This step showed excellent purification yield and resulted in a preparation. The purified enzyme showed a max. activity at pH 7.5 and $50\,^\circ\!C$ and had $K_m$and $V_{\max}$ of 1.876mM and 21.28 mM/h.