Binding Capability and Non-biofouling Efficacy of Poly[2-(methacryloyloxy)ethyl-4-pentynoate-co-oligo(ethylene Glycol) Methacrylate] Films on Gold Surfaces

Abstract

We demonstrated the binding capability and non-biofouling efficacy of biotinylated, poly(MAEP-co-OEGMA) films using streptavidin and fibrinogen. The copolymeric films were synthesized on gold substrates without activation or passivation via the surface initiated-controlled radical polymerization of MAEP and OEGMA in five different molar ratios, followed by biotinylation using "click" chemistry. The copolymeric film synthesized with MAEP at a mole fraction of 0.2 exhibited the best performance of surface plasmon resonance.