DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Rhee, Joon-Shick | - |
dc.contributor.advisor | 이준식 | - |
dc.contributor.author | Kim, Tae-Geun | - |
dc.contributor.author | 김태근 | - |
dc.date.accessioned | 2011-12-12T08:56:33Z | - |
dc.date.available | 2011-12-12T08:56:33Z | - |
dc.date.issued | 1985 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=64492&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/28202 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 생물공학과, 1985.2, [ iii, 43 p. ] | - |
dc.description.abstract | The hydrolysis of triacylglyceride by reversed micelle of the lipase was studied. The enzyme was solubilized in reversed micelle of bis (2-ethylhexyl) sodium sulfosuccinate (25 mM, AOT) in isooctane and water (0.31\%). The enzyme activity depended on the molar ratio of water to AOT (R value), and maximum activity was observed when the ``R value`` was 7. It is remarkable that under the conditions investigated, the reaction rate in the micelle was ca. 6 times faster than that in aqueous emulsion system. The pH-activity profile showed that optimum pH was 7.3 in isooctane-AOT micellar solution. Optimum temperature was at $30-35\,^\circ\!C$. Also, Ea, activation energy was 7.9 Kcal/mole. When ``R`` value was seven, apparent Km and Vmax for olive oil were 0.80\% and 51 uM/mg/hr, respectively. In the enzyme stability against isooctane, the relative activity of the lipase entrapped in AOT-isooctane rapidly dropped to ca. 50\% after 2 hours. The order of the reaction complied with 2nd order reaction. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.title | Hydrolysis of triacylglyceride by the lipase from candida rugosa in isooctane-aot reversed micelle | - |
dc.title.alternative | 이소옥테인-에이.오.티 역 미셀계에서 Candida rugosa 로 부터 얻은 리파-제에 의한 트리글리세라이드의 가수분해 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 64492/325007 | - |
dc.description.department | 한국과학기술원 : 생물공학과, | - |
dc.identifier.uid | 000831109 | - |
dc.contributor.localauthor | Rhee, Joon-Shick | - |
dc.contributor.localauthor | 이준식 | - |
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