Hydrolysis of triacylglyceride by the lipase from candida rugosa in isooctane-aot reversed micelle이소옥테인-에이.오.티 역 미셀계에서 Candida rugosa 로 부터 얻은 리파-제에 의한 트리글리세라이드의 가수분해

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dc.contributor.advisorRhee, Joon-Shick-
dc.contributor.advisor이준식-
dc.contributor.authorKim, Tae-Geun-
dc.contributor.author김태근-
dc.date.accessioned2011-12-12T08:56:33Z-
dc.date.available2011-12-12T08:56:33Z-
dc.date.issued1985-
dc.identifier.urihttp://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=64492&flag=dissertation-
dc.identifier.urihttp://hdl.handle.net/10203/28202-
dc.description학위논문(석사) - 한국과학기술원 : 생물공학과, 1985.2, [ iii, 43 p. ]-
dc.description.abstractThe hydrolysis of triacylglyceride by reversed micelle of the lipase was studied. The enzyme was solubilized in reversed micelle of bis (2-ethylhexyl) sodium sulfosuccinate (25 mM, AOT) in isooctane and water (0.31\%). The enzyme activity depended on the molar ratio of water to AOT (R value), and maximum activity was observed when the ``R value`` was 7. It is remarkable that under the conditions investigated, the reaction rate in the micelle was ca. 6 times faster than that in aqueous emulsion system. The pH-activity profile showed that optimum pH was 7.3 in isooctane-AOT micellar solution. Optimum temperature was at $30-35\,^\circ\!C$. Also, Ea, activation energy was 7.9 Kcal/mole. When ``R`` value was seven, apparent Km and Vmax for olive oil were 0.80\% and 51 uM/mg/hr, respectively. In the enzyme stability against isooctane, the relative activity of the lipase entrapped in AOT-isooctane rapidly dropped to ca. 50\% after 2 hours. The order of the reaction complied with 2nd order reaction.eng
dc.languageeng-
dc.publisher한국과학기술원-
dc.titleHydrolysis of triacylglyceride by the lipase from candida rugosa in isooctane-aot reversed micelle-
dc.title.alternative이소옥테인-에이.오.티 역 미셀계에서 Candida rugosa 로 부터 얻은 리파-제에 의한 트리글리세라이드의 가수분해-
dc.typeThesis(Master)-
dc.identifier.CNRN64492/325007-
dc.description.department한국과학기술원 : 생물공학과, -
dc.identifier.uid000831109-
dc.contributor.localauthorRhee, Joon-Shick-
dc.contributor.localauthor이준식-
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