DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Taehyung C. | ko |
dc.contributor.author | Kang, Minjin | ko |
dc.contributor.author | Kim, Chan Hyuk | ko |
dc.contributor.author | Schultz, Peter G. | ko |
dc.contributor.author | Chapman, Eli | ko |
dc.contributor.author | Deniz, Ashok A. | ko |
dc.date.accessioned | 2021-03-22T09:10:12Z | - |
dc.date.available | 2021-03-22T09:10:12Z | - |
dc.date.created | 2021-02-28 | - |
dc.date.created | 2021-02-28 | - |
dc.date.issued | 2016-06 | - |
dc.identifier.citation | ChemBioChem, v.17, no.11, pp.981 - 984 | - |
dc.identifier.issn | 1439-4227 | - |
dc.identifier.uri | http://hdl.handle.net/10203/281748 | - |
dc.description.abstract | Many cellular functions are critically dependent on the folding of complex multimeric proteins, such as p97, a hexameric multidomain AAA+ chaperone. Given the complex architecture of p97, single-molecule (sm) FRET would be a powerful tool for studying folding while avoiding ensemble averaging. However, dual site-specific labeling of such a large protein for smFRET is a significant challenge. Here, we address this issue by using bioorthogonal azide-alkyne chemistry to attach an smFRET dye pair to site-specifically incorporated unnatural amino acids, allowing us to generate p97 variants reporting on inter-or intra-domain structural features. An initial proof-of-principle set of smFRET results demonstrated the strengths of this labeling method. Our results highlight this as a powerful tool for structural studies of p97 and other large protein machines. | - |
dc.language | English | - |
dc.publisher | John Wiley & Sons Ltd. | - |
dc.title | Dual Unnatural Amino Acid Incorporation and Click-Chemistry Labeling to Enable Single-Molecule FRET Studies of p97 Folding | - |
dc.type | Article | - |
dc.identifier.wosid | 000379156100002 | - |
dc.identifier.scopusid | 2-s2.0-84973137424 | - |
dc.type.rims | ART | - |
dc.citation.volume | 17 | - |
dc.citation.issue | 11 | - |
dc.citation.beginningpage | 981 | - |
dc.citation.endingpage | 984 | - |
dc.citation.publicationname | ChemBioChem | - |
dc.identifier.doi | 10.1002/cbic.201500695 | - |
dc.contributor.localauthor | Kim, Chan Hyuk | - |
dc.contributor.nonIdAuthor | Lee, Taehyung C. | - |
dc.contributor.nonIdAuthor | Kang, Minjin | - |
dc.contributor.nonIdAuthor | Schultz, Peter G. | - |
dc.contributor.nonIdAuthor | Chapman, Eli | - |
dc.contributor.nonIdAuthor | Deniz, Ashok A. | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | INTERPROTOMER MOTION-TRANSMISSION | - |
dc.subject.keywordPlus | PLUS CHAPERONE P97 | - |
dc.subject.keywordPlus | AAA-ATPASE | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | FLUORESCENCE | - |
dc.subject.keywordPlus | MECHANISMS | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.subject.keywordPlus | P97-VCP | - |
dc.subject.keywordPlus | TARGET | - |
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