Effects of T-2 toxin on the primary cultures of adult rat hepatocytes일차배양 간세포 에서의 T-2 독소의 영향에 관한 연구

Cited 0 time in webofscience Cited 0 time in scopus
  • Hit : 338
  • Download : 0
DC FieldValueLanguage
dc.contributor.advisorYang, K. H.-
dc.contributor.advisor양규환-
dc.contributor.authorKim, Hwan-Mook-
dc.contributor.author김환묵-
dc.date.accessioned2011-12-12T08:56:04Z-
dc.date.available2011-12-12T08:56:04Z-
dc.date.issued1984-
dc.identifier.urihttp://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=64049&flag=dissertation-
dc.identifier.urihttp://hdl.handle.net/10203/28169-
dc.description학위논문(석사) - 한국과학기술원 : 생물공학과, 1984.2, [ [iii], 55 p. ]-
dc.description.abstractEffects of T-2 toxin were studied in primary cultures of adult rat hepatocytes. Hepatocytes were isolated by a collagenase perfusion technique and cultured as monolayer in serum-free media for 48 hours. Uptake of cardiac glycoside (ouabain), hormonal induction of $\alpha$-aminoisobutyric acid (AIB) uptake and tyrosine aminotransferase (TAT) activity were used as the parameters to determine cytotoxicity. No significant lethality was observed after 6 hours treatment of 100 ng T-2 toxin/ml. At does from 10 to 100 ng/ml, T-2 toxin inhibited the induction of AIB transport and TAT activity when T-2 toxin was added simultaneously with hormones. It also exerted inhibitory effects when added at 6 hours prior to the hormonal treatment, and removed from medium. Meanwhile, T-2 toxin did not show any inhibitory effects on ouabain transport system. The results of this study suggest that T-2 toxin caused abnormality to liver functions within the testing period, but it does not due to membrane damage and lethal effect. It seems likely that T-2 toxin is a strong inhibitor of protein synthesis in rat hepatocytes.eng
dc.languageeng-
dc.publisher한국과학기술원-
dc.titleEffects of T-2 toxin on the primary cultures of adult rat hepatocytes-
dc.title.alternative일차배양 간세포 에서의 T-2 독소의 영향에 관한 연구-
dc.typeThesis(Master)-
dc.identifier.CNRN64049/325007-
dc.description.department한국과학기술원 : 생물공학과, -
dc.identifier.uid000821093-
dc.contributor.localauthorYang, K. H.-
dc.contributor.localauthor양규환-
Appears in Collection
BS-Theses_Master(석사논문)
Files in This Item
There are no files associated with this item.

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0