DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Dong-Hwa | ko |
dc.contributor.author | Oh, Sohee | ko |
dc.contributor.author | Lim, Kyungeun | ko |
dc.contributor.author | Lee, Boah | ko |
dc.contributor.author | Yi, Gwan-Su | ko |
dc.contributor.author | Kim, Young-Rok | ko |
dc.contributor.author | Kim, Ki-Bum | ko |
dc.contributor.author | Lee, Chong-Kil | ko |
dc.contributor.author | Chi, Seung-Wook | ko |
dc.contributor.author | Lee, Mi-Kyung | ko |
dc.date.accessioned | 2021-03-17T04:50:06Z | - |
dc.date.available | 2021-03-17T04:50:06Z | - |
dc.date.created | 2021-03-17 | - |
dc.date.issued | 2021-02 | - |
dc.identifier.citation | ANALYTICAL CHEMISTRY, v.93, no.5, pp.2811 - 2819 | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.uri | http://hdl.handle.net/10203/281586 | - |
dc.description.abstract | Bacterial riboswitch RNAs are attractive targets for novel antibiotics against antibiotic-resistant superbacteria. Their binding to cognate metabolites is essential for the regulation of bacterial gene expression. Despite the importance of RNAs as therapeutic targets, the development of RNA-targeted, small-molecule drugs is limited by current biophysical methods. Here, we monitored the specific interaction between the adenine-sensing riboswitch aptamer domain (ARS) and adenine at the single-molecule level using alpha-hemolysin (alpha HL) nanopores. During adenine-induced tertiary folding, adenine-bound ARS intermediates exhibited characteristic nanopore events, including a two-level ionic current blockade and a similar to 5.6-fold longer dwell time than that of free RNA. In a proof-of-concept experiment, tertiary RNA folding-targeted drug screening was performed using a protein nanopore, which resulted in the discovery of three new ARS-targeting hit compounds from a natural compound library. Taken together, these results reveal that alpha HL nanopores are a valuable platform for ultrasensitive, label-free, and single-molecule-based drug screening against therapeutic RNA targets. | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | Tertiary RNA Folding-Targeted Drug Screening Strategy Using a Protein Nanopore | - |
dc.type | Article | - |
dc.identifier.wosid | 000618858500013 | - |
dc.identifier.scopusid | 2-s2.0-85100238718 | - |
dc.type.rims | ART | - |
dc.citation.volume | 93 | - |
dc.citation.issue | 5 | - |
dc.citation.beginningpage | 2811 | - |
dc.citation.endingpage | 2819 | - |
dc.citation.publicationname | ANALYTICAL CHEMISTRY | - |
dc.identifier.doi | 10.1021/acs.analchem.0c03941 | - |
dc.contributor.localauthor | Yi, Gwan-Su | - |
dc.contributor.nonIdAuthor | Lee, Dong-Hwa | - |
dc.contributor.nonIdAuthor | Oh, Sohee | - |
dc.contributor.nonIdAuthor | Lim, Kyungeun | - |
dc.contributor.nonIdAuthor | Kim, Young-Rok | - |
dc.contributor.nonIdAuthor | Kim, Ki-Bum | - |
dc.contributor.nonIdAuthor | Lee, Chong-Kil | - |
dc.contributor.nonIdAuthor | Chi, Seung-Wook | - |
dc.contributor.nonIdAuthor | Lee, Mi-Kyung | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
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