Effects of D-galactosamine on primary cultures of adult rat hepatocytes = 일차 배양 간세포 에서의 갈락토사민의 영향에 대한 연구

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Effects of D-galactosamine (GalN) was studied in primary cultures of adult rat hepatocytes. Hepatocytes were isolated by a in situ collagenase perfusion technique and maintained as monolayers in serum free media for 48 hr. To defect the cytotoxicity, effects of GalN on hormonal induction of α-amino isobutyric acid (AIB) transport system and tyrosine aminotransferase (TAT) activity were studied. Effects on uptake of cardiac glycoside, ouabain was also examined. Treatment of GalN inhibited hormonal induction of AIB uptake markedly. At 0.1 mM of GalN, AIB uptake was inhibited significantly when treated for 12 hr. At higher doses (0.25, 0.5 and 1.0 mM) significant inhibition was noticed after 1 hr exposure. Generally the magnitude of the inhibition was dose and treatment time related. Treatment of GalN to the culture also inhibited hormonal induction of TAT activity. The effects was quite similar pattern as in AIB uptake. Meanwhile, uptake of ouabain was not effected by GalN; up to 1 mM of GalN, uptake of ouabain was similar to that of controls. The viability of the hepatocyte was decreased only slightly by the treatment of GalN; more than 87% of the hepatocytes excluded trypan blue when treated 1 mM GalN for 6 hr. GalN-induced depression of AIB uptake and TAT activity was recovered by the addition of uridine to the culture, simultaneously with GalN or after treatment of GalN.
Advisors
Yang, Kyu-Hwan양규환
Description
한국과학기술원 : 생물공학과,
Publisher
한국과학기술원
Issue Date
1983
Identifier
63677/325007 / 000811096
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물공학과, 1983.2, [ [iv], 55 p. ]

URI
http://hdl.handle.net/10203/28146
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=63677&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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