Variable for the production of inulase using jerusalem artichoke media = 돼지 감자 배지를 이용한 이눌라아제 생산에 영향을 미치는 조건에 관한 연구

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$\mbox{\underline{Kluyveromyces}}$ $\mbox{\underline{fragilis}}$ No. 351 was used to study the optimum conditions for inulase production. In order to maximize the production of inulase, the composition of the medium containing Jerusalem artichoke tuber extract were studied. The optimum concentration of Jerusalem artichoke tuber was 3.5\% (dry weight) and bactocasitone was better than yeast extract. The optimum concentration of bactocasitone was 2.0\% and the optimum temperature \& pH of the production of inulase were 30$^\circ$C and pH 5.5 respectively. The addition of $NH_4H_2PO_4$ (0.5\%) increased the production of inulase, and the effect of various metal salt was also studied. The requirement of metal salt was KCl (40 ppm), $MgSO_4\cdot7H_2O$ (20 ppm), $FeSO_4\cdot7H_2O$ (20 ppm), NaCl (20 ppm), $CoCl_2\cdot6H_2O$ (30 ppm). Inulase synthesis was growth - associated (intracellular) and the production of inulase was increased as the concentration of dissolved oxygen concentration was increasing. The addition of Tween 85 increased extracellular inulase but antifoam decreased. Maximum production of inulase was 3.3 unit/ml (Total inulase). A high quantity of industrial grade inulase was prepared by autolysis of the yeast cells, ultrafiltration of supernatant and ethanol fractionation. The recovery was 72\%.
Advisors
Byun, Si-Myung변시명
Description
한국과학기술원 : 생물공학과,
Publisher
한국과학기술원
Issue Date
1982
Identifier
63316/325007 / 000801277
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물공학과, 1982.2, [ viii, 68 p. ]

URI
http://hdl.handle.net/10203/28131
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=63316&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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