Enzyme-linked immunosorbent assay for detection of clostridium botulinum type F toxin = Clostridium botulinum type F toxin 검출의 면역학적 효소방법에 관한 연구

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The enzyme-linked immunosorbent assay using the so-called ``double-sandwich`` technique was applied to determine $\underline{Clostridium}$ $\underline{botulinum}$type F toxin. By this technique, about 10 mouse intraperitoneal 50% lethal doses of type F toxin could be detected with negligible back-ground. This low back-ground reading was achieved with the use of phosphate-buffered saline containing 0.05% Tween 20 and 1% bovine serum albumin (PTB) as a diluent of bridging antibody and conjugate. For accurate determination of type F toxin in samples, addition of EDTA seems to be necessary. This assay permits no cross-reaction with botulinum toxins of other types tested.
Advisors
Yang, Kyu-Hwan양규환
Description
한국과학기술원 : 생물공학과,
Publisher
한국과학기술원
Issue Date
1982
Identifier
63310/325007 / 000801214
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물공학과, 1982.2, [ [vi], 50 p. ]

URI
http://hdl.handle.net/10203/28125
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=63310&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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