Biosynthesis od cephaloglycine using whole cell system = 균체효소를 이용한 세팔로그라이신 합성

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Cephaloglycin (CEG) was synthesized directly from D-α-phenylglycine methyl ester (PGM) and 7-amino cephalospolanic acid (7-ACA) by whole cell enzyme of the $\mbox{\underline{Xanthomonas}}$ $\mbox{\underline{citri}}$ IFO 3835. To data, cephaloglycin has been manufactured by chemical process involving fairly large number of steps to protect other function group. However, the enzymatic process involves only a single step. The production of CEG with X. citri was identified by the bioassay, the paper chromatography, and the HPLC. The reaction model of CEG synthesis and the inhibition of the product of PG were studied through HPLC. The kinetic constants of PGM hydrolysis, CEG synthesis, and CEG hydrolysis were determined. The $K_m$ values of PGM 7-ACA, and CEG were 16 mM, 30 mM, and 167 mM, and $K_i$ value of PG was 20-22 mM. The optimum condition to synthesize CEG was studied, and the method (enzyme loading and solvent effect) to increase the CEG synthesis was researched.
Advisors
Ryu, Doo-Young유두영
Description
한국과학기술원 : 생물공학과,
Publisher
한국과학기술원
Issue Date
1982
Identifier
63306/325007 / 000801131
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물공학과, 1982.2, [ vii, 67 p. ]

URI
http://hdl.handle.net/10203/28121
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=63306&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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