Transcriptomic analysis of Chlorella sp. HS2 suggests the overflow of acetyl-CoA and NADPH cofactor induces high lipid accumulation and halotolerance

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Previously, we isolated Chlorella sp. HS2 (referred hereupon as HS2) from a local tidal rock pool and demonstrated its halotolerance and high biomass productivity under different salinity conditions. To further understand acclimation responses of this alga under high salinity stress, we performed transcriptome analysis of triplicated culture samples grown in freshwater and marine conditions at both exponential and stationary growth phases. The results indicated that the transcripts involved in photosynthesis, TCA, and Calvin cycles were downregulated, whereas the upregulation of DNA repair mechanisms and an ABCB subfamily of eukaryotic type ABC transporter was observed at high salinity condition. In addition, while key enzymes associated with glycolysis pathway and triacylglycerol (TAG) synthesis were determined to be upregulated from early growth phase, salinity stress seemed to reduce the carbohydrate content of harvested biomass from 45.6 dw% to 14.7 dw% and nearly triple the total lipid content from 26.0 dw% to 62.0 dw%. These results suggest that the reallocation of storage carbon toward lipids played a significant role in conferring the viability of this alga under high salinity stress by remediating high level of cellular stress partially resulted from ROS generated in oxygen-evolving thylakoids as observed in a direct measure of photosystem activities.
Publisher
Wiley
Issue Date
2021-02
Language
English
Article Type
Article
Citation

Food and Energy Security, v.10, no.1, pp.e267

ISSN
2048-3694
DOI
10.1002/fes3.267
URI
http://hdl.handle.net/10203/281169
Appears in Collection
CBE-Journal Papers(저널논문)
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