Characterization of drosophila PARK8 = 초파리 PARK8 유전자의 기능 연구

Cited 0 time in webofscience Cited 0 time in scopus
  • Hit : 242
  • Download : 0
Drosophila PARK8(DPARK8) gene is a Drosophila homologue of human LRRK2 whose mutation causes autosomal dominant parkinsonism. DPARK8 gene consists of 7056 nucleotides with twelve exons and encodes 2351 amino acids protein containing ankyrin, LRR, ROC, and kinase domains conserved with its mammalian counterpart. Here, I generated and characterized a loss-of-function mutant and gain-of-function mutants for DPARK8 to investigate its physiological function. By using piggy bag transposable element insertion mutant as a loss-of-function mutant, immunohistochemistry and behavioral assays were applied. The defects in the gross structure of the neuromuscular system were not observed while fertility and fecundity were significantly reduced in the mutant females, which became severer with age. Also, the motor abilities of this mutants quantified by climbing assay were markedly decreased compared to wild type controls. Then, I examined the mitochondrial structures, muscle actin fibre arrays and the number of TH-positive neurons to further characterize the mutant phenotypes, but no significant defects were observed. In addition, overexpression of $DPARK8^{WT}$ and $DPARK8^{KR}$ by gmr-GAL4 and elav-GAL4 drivers caused degenerated eye phenotype and defective wing and incomplete cuticle formation, respectively. Taken together, using Drosophila system, I developed invaluable tools to study the pathogenic mechanism behind autosomal dominant parkinson’s disease (AD-PD) and established novel readout for future studying of parkinsonism.
Advisors
Chung, Jong-kyeongresearcher정종경researcher
Description
한국과학기술원 : 생명과학과,
Publisher
한국과학기술원
Issue Date
2007
Identifier
301301/325007  / 020053960
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생명과학과, 2007.2, [ v, 49 p. ]

Keywords

parkinson\`s disease; 파킨슨병

URI
http://hdl.handle.net/10203/28100
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=301301&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
Files in This Item
There are no files associated with this item.

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0